New technique wins prize for understanding gut health

For his work in developing Record-Seq, a breakthrough technology that non-invasively records the activities of the microbiota in the gut and advances the understanding of gastrointestinal health, Florian Schmidt is the 2022 grand prize winner. science and the SciLifeLab Prize for Young Scientists.

Schmidt’s essay describes engineering cells Escherichia coli ,e coli ), a bacterium commonly found in the human gut, acts as a sentry that travels through the gastrointestinal tract and records in its DNA the transcriptional response to various interactions between itself and the host. Unlike RNA, which is relatively short-lived and fragile, DNA produced by e coli Sentinel cells record information from different time points that can be efficiently and non-invasively retrieved from host feces by deep sequencing.

“Schmidt’s work is highly creative and a technological milestone. Following the path from concept to realization, his research led to the development of a unique method that allows bacterial sensor cells to detect changes in their host’s gut.” Tracking the response of interactions between different microbiota and the host could greatly improve our understanding of the impact of the microbiome on health and disease,” said Sacha Vignieri, Deputy Editor science,

Inspired by the idea of ​​reprogramming the adaptive microbial immune system CRISPR, Schmidt and his colleagues took advantage of the CRISPR spacer acquisition complex to develop Record-Seq, using it as DNA to report on conditions within the gut. Optimized to store and record transcriptional reactions.

best practice creation

Schmidt said that while a new technology, Record-Seq was not originally intended as a non-invasive tool to interrogate the gut microbiome. But once its full potential to record the cellular history of bacteria was discovered, he and his colleagues Tanmay Tanna and Jakob Zimmermann from the laboratories of Randall Platt at ETH Zurich and Andrew McPherson at Bern University Hospital recorded— Worked to seek. Microbiome.

Current methods of examining intestinal tracts include invasive surgery to measure the activity of the intestine, or stool- and blood-based tests that do not report on specific conditions within the intestine. record-seek-derivative e coli The cells present a lens into the adaptation and behavior of proximal sections of the gut and the microbiota so that they can be examined non-invasively.

“Other scientists have previously developed biosensors that can sense the presence of a specific small molecule … yet they are fine-tuned to sense the presence or absence of a small group of molecules, but not much else.” are blind to,” Schmidt explained. “With Record-Seq, we remove this proverbial blindfold … anything that interacts with bacteria and that changes their behavior can be captured.”

Hopefully, researchers can now use Record-Seq as a tool to find new biomarkers related to nutrition and disease, Schmidt noted. In the future, this may enable further translational efforts in diagnostics and therapeutics.

future of gut health

Schmidt and his colleagues fed these to rats e coli cells to test the recording of transcriptional information inside the intestine. The group was able to recover this information by DNA-sequencing cells from the mice’s feces, prompting the researchers to make changes to the mice’s diets that were being recorded.

record-seek e coli for different diets and to maintain this information. While both RNA-sequencing (RNA-seq) and Record-seq could differentiate between a standard chow or starch diet, only Record-seq retained information from the earlier diet after a switch. Record-seq also retains information about the length of the gastrointestinal tract, whereas much of the information is lost from faecal RNA-seq samples.

These insights into the hidden life of the microbiota inside the gut are fascinatingly complex, and Record-Seq opens a new window for understanding how nutrition, inflammation and microbial interactions shape health and disease inside the gastrointestinal tract.

“Think of all the diseases and interactions your gut is involved in and consider also that we can use this to test how humans interact with their diet. Different diets have different responses. The response of individual individuals is surprising and may even contribute to disease conditions such as malnutrition or obesity. Record-Seq could be deployed to investigate and inform treatment decisions in these diseases as well as food intolerance, Schmidt said. Is.

science & SciLifeLab Awards for Young Scientists recognizes that global economic health depends on a vibrant research community, which needs to encourage the best and brightest to continue in their chosen areas of research as they begin their scientific careers. the wanted. The grand prize winner receives an award of $30,000.

“Over the years, what’s striking about the winners of science & SciLifeLab Prize their ability to clearly explain their exciting scientific discoveries and to place them in the wider context of biology, medicine, and even immediate societal challenges. This is also the case this year, with three essays describing a variety of research on microbes and one on the regulation of proteins involved in cell growth in health and disease,” said Oli Kalionemi, director of SciLifeLab. “At SciLifeLab we Excited to take. Participate in this award and hope that this recognition will fuel the careers of these next generation of research leaders and make their research widely known. Congratulations to all four winners of this year’s award.”

2022 recipient

Stephanie Moreno-Gemez | Deianira Games

stephanie moreno-gamez His essay, “How Bacteria Navigate in Different Environments,” is a winner. Moreno-Gámez graduated from the Universidad de los Andes and holds a Ph.D. from the University of Groningen and ETH Zurich. He is currently a postdoctoral fellow at the Massachusetts Institute of Technology. His research focuses on how diet and host-derived glycans shape ecological and evolutionary dynamics in the gut microbiome.

Headshot of James L. Daly
James L Daly

James L Daly There is a winner for his essay “Endosomes, Receptors and Viruses”. Daly holds a graduate and Ph.D. degree from the University of Bristol. After completing his studies, he received a Wellcome Early Career Award fellowship and moved to the Department of Infectious Diseases at King’s College London. His current research continues to explore the molecular interface between neuropilin receptors and viruses and the potential for antiviral inhibition of this process.

Daniel Simonesky There is a winner for their essay, “Unraveling the Degrador of D-Type Cyclins”.

Headshot of Daniel Simonsky
Daniel Simonesky

Simonschi received his undergraduate degree from Manhattanville College and his MPhil and Ph.D. degree from the Vilseck Institute at New York University. He is a research assistant professor in the Department of Biochemistry and Molecular Pharmacology at NYU, where he researches the molecular and cellular mechanisms by which cullin-RING ubiquitin ligases regulate cell cycle execution.

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